Three-parent baby is an in vitro fertilization technique that involves combination of DNA from 3 parents to produce a baby. In this technique mitochondrial DNA is taken from the donor while nuclear DNA is from biological parents.
Three-parent in vitro fertilization (TPIVF) can be performed with 2 techniques:
A. Pronuclear Transfer:
In this technique nucleus from the egg (with diseased mitochondrial DNA) of the female parent is extracted and placed in the donor egg with healthy mitochondrial DNA. This hybrid egg is then fertilized with sperm. Resulting embryo will have 100% nuclear DNA from biological parents, while over all genetic composition will be 99.9% from parents and 0.1% from donor. However 3 genetic parents can still be identified on genetic analysis.
B. Spindle Transfer:
In this technique spindle from the mother’s chromosome are extracted and placed in the donor’s egg.